GENHET: this function for R calculates the five most important individual heterozygosity estimates all at once without any size limitation (loci, alleles or individuals). It is very easy to use, even people who have never used R should be able to make it work. Click here to get the function, its manual and example files.
ALF: another easy-to-use function for R; it estimates allele frequencies from a genotype file. Click here to get the function and example files.
